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BIOT2225 – Biotechnology Methods II pdf

Credits: 4 (2/2/0)
Description: This course is the second in a two-semester sequence designed to train laboratory technicians in the fundamental technologies, procedures and processes utilized within the biotechnology industry. This course emphasizes technical skills development, record-keeping and communication skills, and conformity to good laboratory and good manufacturing practices (GLPs/GMPs). This course meets for two hours of lecture and four hours of laboratory each week.
Prerequisites: BIOT2215
Corequisites: (None)
  1. Perform record-keeping, communication and technical skills that conform to industry standards.
  2. Compare and contrast various DNA sequencing technologies.
  3. Discuss the utility of bioinformatics software in determining sequence homologies.
  4. Describe microarray construction, application and data analysis.
  5. Discuss features of bacterial and mammalian expression vectors.
  6. Summarize methods used to visualize and quantitate proteins.
  7. Explain chromatography techniques used to characterize proteins.
  8. Discuss native and denaturing polyacrylamide gel electrophoresis (PAGE).
  9. Summarize methodologies involved in monoclonal and polyclonal antibody production.
  10. Discuss the use of antibodies in immunoprecipitation, immunoblotting and immunohistochemical analysis.
  11. Analyze experimental results.
  12. Troubleshoot experiments and equipment.
  13. Discuss emerging trends in biotechnology and molecular therapeutics.
MnTC goal areas: (N/A)

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