||This course is the second in a two-semester sequence designed to train laboratory technicians in the fundamental technologies, procedures and processes utilized within the biotechnology industry. This course emphasizes technical skills development, record-keeping and communication skills, and conformity to good laboratory and good manufacturing practices (GLPs/GMPs). This course meets for two hours of lecture and four hours of laboratory each week.
- Perform record-keeping, communication and technical skills that conform to industry standards.
- Compare and contrast various DNA sequencing technologies.
- Discuss the utility of bioinformatics software in determining sequence homologies.
- Describe microarray construction, application and data analysis.
- Discuss features of bacterial and mammalian expression vectors.
- Summarize methods used to visualize and quantitate proteins.
- Explain chromatography techniques used to characterize proteins.
- Discuss native and denaturing polyacrylamide gel electrophoresis (PAGE).
- Summarize methodologies involved in monoclonal and polyclonal antibody production.
- Discuss the use of antibodies in immunoprecipitation, immunoblotting and immunohistochemical analysis.
- Analyze experimental results.
- Troubleshoot experiments and equipment.
- Discuss emerging trends in biotechnology and molecular therapeutics.
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